Profiling humoral immune responses to P. falciparum infection with protein microarrays

Profiling the immune response to TB infection on a genome-wide scale with protein microarrays

The process for fabrication of a microarray from genomic DNA for whole proteome microarrays for B. pseudomallei may have application for Mycobacterium tuberculosis (M.tb). In the process, PCR is done on the DNA to amplify the open reading frames, genes are cloned by in vivo recombination, and mini-preps are made, isolating the plasmids produced by the recombination process. Then, in vitro trans...

Humoral immune responses after rabies infection.

Identification of humoral immune responses in protein microarrays using DNA microarray data analysis techniques

MOTIVATION We present a study of antigen expression signals from a newly developed high-throughput protein microarray technique. These signals are a measure of antibody-antigen binding activity and provide a basis for understanding humoral immune responses to various infectious agents and supporting vaccine and diagnostic development. RESULTS We investigate the characteristics of these expres...

Profiling the humoral immune response to infection by using proteome microarrays: high-throughput vaccine and diagnostic antigen discovery.

Despite the increasing availability of genome sequences from many human pathogens, the production of complete proteomes remains at a bottleneck. To address this need, a high-throughput PCR recombination cloning and expression platform has been developed that allows hundreds of genes to be batch-processed by using ordinary laboratory procedures without robotics. The method relies on high-through...

Maternal and neonatal immune responses to Plasmodium falciparum infection

Plasmodium falciparum infection during pregnancy has severe consequences for both mother and fetus and can lead to the trans-placental passage of malarial antigens that are capable of inducing neonatal immune responses. Between July 2003-December 2004, 228 women from Omdurman Maternity Hospital, Sudan were followed monthly during antenatal period till delivery time. We examined neonatal and mat...